Biotechnological studies on Arthrospira platensis biomass cultivated in enriched culture with chelated leather waste and chelated glycinate

Abstract The current work aims to evaluate the growth rate, molecular patterns and biological activities of Arthrospira platensis cultivated in culture enriched with different chelated minerals (Fe, Zn, Cu, Mn, Mg) leather protein hydrolysates (LPHs) glycine addition determine phytochemical contents modified Zarrouk medium. Also, study profile variations (using SDS-PAGE, Comet assay, SCOT-PCR) collected biomasses when compared control (cyanobacteria medium). Superoxide dismutase (SOD), glutathione peroxidase (GPX), mineral content each treatment were also determined. obtained Results showed that a significant increment rate A. glycinate treatments was observed especially Cu-glycinate more than Additionally, T11 has high O.D. other but medium had highest LW-chelated minerals. All treated glycine-chelates possessed both conserved additional proteins their SDS-PAGE banding patterns. Moreover, by waste hydrolysate induced discrimination analogues glycine. Six ScoT primers applied produced 323 amplicons an average 68% polymorphism/primer. SDS Scot dendrogram revealed among all treatments, Mg-glycine-chelated closely related. samples polymorphic bands monomorphic ones (either as or DNA), despite no DNA damage (as % tDNA) detected on either treatment.